Composition and functions of rumen and endometrial microorganisms associated with endometritis in dairy cows

Despite the attention given in recent years to microbiological causes of endometritis and potential sources uterine microbiota infestation, more questions than answers remain this research field. This paper describes an experiment carried out at livestock farm Leningrad region on 6 dairy cows Holsteinized Russian Black Pied breed second lactation period after calving. The animals were divided into two groups (n = 3): Group 1 included clinically healthy animals, 2 diagnosed with purulent-catarrhal post-calving endometritis. Metagenomic sequencing was performed using MiSeq genomic sequencer (Illumina, Inc., USA) Reagent Kit v3 USA). Based next-generation endometrial scrapings, 7 phyla microorganisms detected only 4 increase proportion Fusobacteriota taxon bacteria permanently present scrapings decrease Bacteroidota phylum group could be related occurrence disease. Bacteria Alloprevotella, Campylobacter, Caviibacter, Falsiporphyromonas, Veillonella tissue sick may etiological origin In rumen endometritis, there (p≤0.05) against a Firmicutes compared group. Using PICRUSt2 software package (v.2.3.0), it shown that microbiome showed inhibition 9 metabolic pathways animals. have negative consequences for body consequence disorders.